Journal: Journal of visualized experiments : JoVE
Article Title: Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
doi: 10.3791/62163
Figure Lengend Snippet: Materials
Article Snippet: Name Company Catalog Number Comments bovine serum albumin (BSA) Gold Biotechnology A-420-100 C57BL/6 mouse (C57BL/6J) The Jackson Laboratory 000664 collagenase (Collagenase A) Sigma-Aldrich 10103586001 culture dish (35 mm in diameter) Genesee Scientific 32-103G culture dish (100 mm in diameter) Genesee Scientific 32-107G dispase (Dispase II) Sigma-Aldrich 04942078001 dissecting scissors (Student Fine Scissors) Find Science Tool 91460-11 DMEM/F12 Gibco 11320033 fetal bovine serum (FBS) Hyclone C838U82 fine forceps (Dumount #3 Forceps) Find Science Tool 11293-00 hemocytometer Hausser Scientific 3520 inverted microscope with imaging system (EVOS XL Core Cell Imaging System) Life Technologies AMEX1000 low retention pipette tips METTLER TOLEDO 17014342 mini-scissors (Evo Spring Scissors) Fine Science Tool 15800-01 plastic warp VWR 46610-056 spatula (Moria Spoon) Fine Science Tool 10321-08 surgical forceps (Dumount #2 Laminectomy Forceps) Fine Science Tool 11223-20 Trypan blue Gibco 15250061 Tyrode’s solution Sigma-Aldrich T2145-10L made from Tyrode’s salts 0.25% typsin-EDTA Gibco 25200056 0.1 M Phosphate-Buffered Saline (PBS) Hoefer 33946 made from 1 M PBS 0.22-μm syringe filter Genesee Scientific 25-243 70% ethanol Koptec 233919 made from 100% ethanol 1-mL syringe BD 8194938 5-mL low binding microcentrifuge tube Eppendorf 30122348 30-G needle BD 9193532 35-μm cell strainer Falcon 64750 70-μm cell strainer Falcon 64752 Open in a separate window Materials.
Techniques: Inverted Microscopy, Imaging, Transferring, Saline, Binding Assay